Regulatory

Part:BBa_K5371010:Design

Designed by: Haoyang Yu   Group: iGEM24_iZJU-China   (2024-09-28)


pHXT1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 120
    Illegal SpeI site found at 779
    Illegal PstI site found at 423
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 120
    Illegal SpeI site found at 779
    Illegal PstI site found at 423
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 120
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 120
    Illegal SpeI site found at 779
    Illegal PstI site found at 423
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 120
    Illegal SpeI site found at 779
    Illegal PstI site found at 423
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 727


Design Notes

The pHXT1 part in prs42b-erg9 plasmid acts as a weak promoter. The pERG9-down and pERG9-up are located at opposite ends of the pHXT1 plasmid and are responsible for precise integration into the ERG9 gene region through homologous recombination. The pHXT1 indirectly enhances the effect of the MrBBS-ERG20 plasmid by weakening ERG9 expression, allowing more FPP to be used for the synthesis of α-bisabolol rather than to squalene.


Source

The pHXT1 was amplified from genome of Saccharomyces cerevisiae.

References